| # | Name | Team | Age | Pos | Level |
| 1 | Juan Soto | Washington | 20 | LF | MLB |
| 2 | Vladimir Guerrero Jr. | Chicago | 19 | 3B | MLB |
| 3 | Ronald Acuna Jr. | Boston | 21 | CF | MLB |
| 4 | Shohei Ohtani | San Diego | 24 | RF | MLB |
| 5 | Walker Buehler | LA Angels | 24 | SP | MLB |
| 6 | Gleyber Torres | LA Angels | 22 | 2B | MLB |
| 7 | Forrest Whitley | Boston | 21 | SP | AAA |
| 8 | Nick Senzel | Chicago | 23 | 3B | MLB |
| 9 | Mike Soroka | LA Dodgers | 21 | SP | MLB |
| 10 | German Marquez | Seattle | 24 | SP | MLB |
| 11 | Shane Bieber | Atlanta | 23 | SP | MLB |
| 12 | Fernando Tatis Jr. | Oakland | 20 | 3B | AA |
| 13 | Eloy Jimenez | NY Mets | 22 | LF | AAA |
| 14 | Jordan Hicks | LA Dodgers | 22 | CL | MLB |
| 15 | MacKenzie Gore | LA Angels | 19 | RP | MLB |
| 16 | Willy Adames | LA Angels | 23 | SS | MLB |
| 17 | Wander Samuel Franco | Boston | 17 | SS | A |
| 18 | Eric Pardinho | Boston | 18 | SP | R |
| 19 | Brent Honeywell | Boston | 23 | SP | MLB |
| 20 | Joey Bart | Oakland | 22 | C | A |
| 21 | Victor Robles | Chicago | 21 | CF | MLB |
| 22 | Caleb Ferguson | Houston | 22 | RP | MLB |
| 23 | Kyle Tucker | Atlanta | 22 | LF | MLB |
| 24 | Royce Lewis | Philadelphia | 19 | SS | A |
| 25 | Francis Martes | Boston | 23 | SP | A |
| 26 | A.J. Puk | Washington | 23 | RP | MLB |
| 27 | Carter Kieboom | Boston | 21 | 3B | AA |
| 28 | Luis Urias | LA Dodgers | 21 | 2B | MLB |
| 29 | Casey Mize | Chicago | 21 | RP | MLB |
| 30 | Francisco Mejia | Boston | 23 | C | MLB |
| 31 | Joey Lucchesi | Houston | 25 | RP | MLB |
| 32 | Brendan Rodgers | Boston | 22 | 2B | MLB |
| 33 | Taylor Trammell | St. Louis | 21 | CF | AA |
| 34 | Chris Paddack | Houston | 23 | RP | MLB |
| 35 | Dakota Hudson | Houston | 24 | RP | MLB |
| 36 | Miguel Amaya | Boston | 19 | C | R |
| 37 | Jo Adell | LA Dodgers | 19 | CF | A |
| 38 | Ian Anderson | Seattle | 20 | SP | A |
| 39 | Jesus Luzardo | Boston | 21 | SP | AAA |
| 40 | Tyler O’Neill | Miami | 23 | RF | MLB |
| 41 | Nick Madrigal | Chicago | 21 | SS | A |
| 42 | Jack Flaherty | St. Louis | 23 | SP | MLB |
| 43 | Miguel Andujar | Washington | 23 | 3B | MLB |
| 44 | Franmil Reyes | Washington | 23 | RF | MLB |
| 45 | Sixto Sanchez | NY Yankees | 20 | RP | MLB |
| 46 | Corbin Burnes | Chicago | 24 | RP | MLB |
| 47 | Alex Kirilloff | San Diego | 21 | RF | A |
| 48 | Bo Bichette | Detroit | 20 | 2B | MLB |
| 49 | Freddy Peralta | Philadelphia | 22 | RP | MLB |
| 50 | Michael Kopech | Chicago | 22 | RP | MLB |
| 51 | Amed Rosario | Boston | 23 | SS | MLB |
| 52 | Alec Bohm | Detroit | 22 | 1B | S A |
| 53 | Michel Baez | St. Louis | 23 | RP | MLB |
| 54 | Estevan Florial | Atlanta | 21 | CF | MLB |
| 55 | Dylan Cozens | St. Louis | 24 | LF | MLB |
| 56 | Adrian Morejon | Boston | 19 | SP | AA |
| 57 | Hunter Greene | Houston | 19 | SP | R |
| 58 | Danny Jansen | Seattle | 23 | C | MLB |
| 59 | Oneil Cruz | LA Dodgers | 20 | 1B | S A |
| 60 | Dylan Cease | Boston | 23 | SP | AA |
| 61 | Triston McKenzie | Seattle | 21 | SP | A |
| 62 | Cristian Pache | Seattle | 20 | CF | A |
| 63 | Jonathan India | LA Angels | 22 | 3B | MLB |
| 64 | Fernando Romero | Boston | 24 | RP | AAA |
| 65 | Franchy Cordero | Boston | 24 | LF | MLB |
| 66 | Austin Riley | LA Dodgers | 21 | 3B | AAA |
| 67 | Matt Manning | St. Louis | 21 | SP | AA |
| 68 | Brad Keller | Houston | 23 | SP | MLB |
| 69 | Clint Frazier | Oakland | 24 | LF | MLB |
| 70 | Keibert Ruiz | Seattle | 20 | C | MLB |
| 71 | Justus Sheffield | NY Mets | 22 | RP | MLB |
| 72 | Sean Murphy | Boston | 24 | C | AA |
| 73 | Keston Hiura | Boston | 22 | 2B | AA |
| 74 | Brady Singer | Atlanta | 22 | SP | S A |
| 75 | Brandon Woodruff | Chicago | 26 | SP | MLB |
| 76 | Alex Verdugo | LA Dodgers | 22 | LF | MLB |
| 77 | Jake Bauers | LA Angels | 23 | LF | MLB |
| 78 | Brusdar Graterol | San Diego | 20 | SP | S A |
| 79 | Taylor Widener | Oakland | 24 | RP | MLB |
| 80 | Jesus Armando Sanchez | LA Dodgers | 21 | CF | A |
| 81 | Vidal Brujan | St. Louis | 21 | 2B | A |
| 82 | Mitch Keller | Chicago | 22 | RP | MLB |
| 83 | Austin Meadows | Washington | 23 | CF | MLB |
| 84 | Luis Rengifo | LA Dodgers | 21 | 2B | A |
| 85 | Dustin May | Seattle | 21 | SP | A |
| 86 | Luiz Gohara | Boston | 22 | RP | AA |
| 87 | Andres Gimenez | Seattle | 20 | SS | A |
| 88 | Jazz Chisholm | San Diego | 21 | SS | A |
| 89 | Jonathan Loaisiga | LA Dodgers | 24 | RP | MLB |
| 90 | Franklin Perez | LA Dodgers | 21 | RP | MLB |
| 91 | Harrison Bader | San Diego | 24 | LF | MLB |
| 92 | Austin Beck | Seattle | 20 | CF | R |
| 93 | Anthony Banda | Boston | 25 | RP | AA |
| 94 | Trevor Larnach | Seattle | 21 | RF | A |
| 95 | Bubba Thompson | Boston | 20 | RF | S A |
| 96 | Kyle Wright | NY Mets | 23 | RP | A |
| 97 | Cionel Perez | Seattle | 22 | RP | MLB |
| 98 | Yordan Alvarez | Boston | 21 | RF | AA |
| 99 | Daulton Varsho | Chicago | 22 | C | A |
| 100 | Luis Patino | Oakland | 19 | SP | R |
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